Regulatory

Part:BBa_K4226003

Designed by: Siyu Han   Group: iGEM22_CAFA_China   (2022-09-19)


hrpL Promoter

This part is an improvement for the part：Promoter hrpL uploaded by iGEM13_HIT-Harbin. This promoter will be active, when both hrpR Gene and hrpS Gene are present. The hrpL Promoter is transcriptionally upregulated by hrpR and hrpS. The Amp30E Amplification Device consists of hrpR Gene, hrpS Gene and hrpL promoter.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



Reference
[1] Wan, Xinyi, et al. "Cascaded amplifying circuits enable ultrasensitive cellular sensors for toxic metals." Nature chemical biology 15.5 (2019): 540-548.

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Parameters
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